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题目
DNA提取中加入无水乙醇,有明显的丝状DNA出现 高速离心沉淀后DNA却不见了
请问大家这是怎么回事?是dna又溶解了吗
但确实是看不见了,不可能是透明的,正常应该是白色,这到底怎么回事

提问时间:2020-10-28

答案
You have to think about the solubility of DNA in water and ethanol.The surface of DNA is highly charged and as a result a polar solution will dissolve DNA.Water is more polar then ethanol,so DNA has higher solubility in water than in ethanol.
When you put in some ethanol that is not evenly mixed with water,DNA precipitates.But when water and ethanol are mixed again,DNA is dissolved.
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