题目
Our investigations continued with determination of the stability and reactivity of the two componen
Our investigations continued with determination of the
stability and reactivity of the two components in biological
media.In vitro assays in PBS,serum,and blood showed
(Table 1) that [111In]-1 should be stable for the duration of its
presence in mice (blood clearance half-life of 9.8 minutes; see
Figure S13 in the Supporting Information).We observed a
fast reaction between [111In]-1 and CC49-TCO in vitro in
semi-equimolar conditions and at low concentration (3.3 mm)
within 10 minutes in PBS,serum and blood (Table 2).The
yields obtained from the reactions with 10 and 15 equivalents
of [111In]-1 indicated the presence of an average of 7.4 reactive
TCO moieties per antibody.Furthermore,the nonspecific
binding of [111In]-1 to unmodified CC49 or other media
constituents was found to be very low.To assess the stability
of the mAb-boun
Our investigations continued with determination of the
stability and reactivity of the two components in biological
media.In vitro assays in PBS,serum,and blood showed
(Table 1) that [111In]-1 should be stable for the duration of its
presence in mice (blood clearance half-life of 9.8 minutes; see
Figure S13 in the Supporting Information).We observed a
fast reaction between [111In]-1 and CC49-TCO in vitro in
semi-equimolar conditions and at low concentration (3.3 mm)
within 10 minutes in PBS,serum and blood (Table 2).The
yields obtained from the reactions with 10 and 15 equivalents
of [111In]-1 indicated the presence of an average of 7.4 reactive
TCO moieties per antibody.Furthermore,the nonspecific
binding of [111In]-1 to unmodified CC49 or other media
constituents was found to be very low.To assess the stability
of the mAb-boun
提问时间:2021-04-21
答案
我们的研究随着确定在生物媒质中两种成分的稳定性和反应性而继续.在PBS、血清和血中的体外化验表明(表1)[IIIIn]-1对于其存在于老鼠中的持续时间(血液清除率半衰期为9.8min;见支持信息中的图S13)来说是稳定的.我...
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