题目

英语翻译
Transcription-coupled DNA repair uses components of the transcription machinery to identify DNA lesions and initiate their repair.These repair pathways are complex,so their mechanistic features remain poorly understood.Bacterial transcription-coupled repair is initiated when RNA polymerase stalled at a DNA lesion is removed by Mfd,an ATP-dependent DNA translocase1,2,3.Here we use single-molecule DNA nanomanipulation to observe the dynamic interactions of Escherichia coli Mfd with RNA polymerase elongation complexes stalled by a cyclopyrimidine dimer or by nucleotide starvation.We show that Mfd acts by catalysing two irreversible,ATP-dependent transitions with different structural,kinetic and mechanistic features.Mfd remains bound to the DNA in a long-lived complex that could act as a marker for sites of DNA damage,directing assembly of subsequent DNA repair factors.These results provide a framework for considering the kinetics of transcription-coupled repair in vivo,and open t

提问时间：2021-03-22

答案

DNA转录偶联修复使用的转录机器识别DNA损伤,并启动其维修的组件.这些修复途径很复杂,所以机械功能仍知之甚少.RNA聚合酶的DNA病变停滞在启动时的ATP-依赖的DNA反禄ASE1,2,3的MFD,除去细菌转录偶联修复.在这里,我们使...