题目

英语翻译
Results
Detection of upregulated proteins
In order to identify membrane proteins of K562 cells with
increased expression under iron deprivation,we isolated
hydrophobic membrane proteins from the cells after 24-h
incubation under control conditions (defined transferrin
medium supplemented with 5 lg/ml of transferrin as the
source of iron) and under iron deprivation (defined ironfree
medium without any added source of iron,see Materials
and methods) using a two-phase partitioning system
(2-D Sample Prep for Membrane Proteins from Pierce,see
Materials and methods).We prepared three pairs of 2D gels
from three separate pairs of samples of control cells and
cells cultured under iron deprivation.Gels were stained
using colloidal Coomassie blue.After scanning the stained
gels in a densitometer,we analyzed triplets of gel images
using PDQuest 7.1 2-D Analysis Software in semimanual
detecting and matching mode.
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提问时间：2020-08-08

答案

结果检测上调的蛋白质为了确定膜蛋白的K562细胞与表达增加铁剥夺下,我们孤立疏水膜蛋白的细胞后24小时孵化控制条件下（定义转培养基5 LG电子/毫升的转来源铁）和铁剥夺（定义ironfree 中期没有任何补充铁的来源,见材...